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  • Quantitative detection of Escherichia coli from urine

    2012-12-1 · In the present study we used real-time PCR to make a quantitative analysis of E. coli in the urine of patients with UTI. Real-time PCR allows quantification of a targeted gene when the sequence of the targeted gene is known. Since uspA was found to be highly specific for E. coli, we used primers and probes derived from this gene in our experiments. Since the genetic sequences of so many bacteria are now known, primers and probes specific to each can …

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  • Separating and Detecting Escherichia Coli in a ...

    2011-8-12 · In this thesis, I present a lab-on-a-chip (LOC) that can separate and detect Escherichia Coli (E. coli) in simulated urine samples for Urinary Tract Infection (UTI) diagnosis. The LOC consists of two (concentration and sensing) chambers connected in series and an integrated impedance detector. The two-chamber approach is designed to reduce the non-

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  • E. coli (Escherichia coli) | E. coli | CDC

    2020-2-26 · E. coli are a large and diverse group of bacteria. Although most strains of E. coli are harmless, others can make you sick. Some kinds of E. coli can cause diarrhea, while others cause urinary tract infections, respiratory illness and pneumonia, and other illnesses.

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  • Sensitive and rapid detection of pathogenic bacteria

    2010-7-27 · Drugs like cocaine, ecstasy or heroin can show up in test results for up to five days. Various types of tests can be used here too: Rapid tests help give police fast results on site, while other drug tests need to be sent to a laboratory. Urine samples can also be used to test athletes for banned performance-enhancing substances (doping).

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  • Understanding urine tests - InformedHealth.org -

    2012-2-6 · The urine from normal person was spiked with uropathogenic Escherichia coli isolated from urinary tract infected patient. To prepare urine spiked clinical samples, 10 6 - 10 5 cfu/ml Escherichia coli were added to one ml of urine and centrifuged at 14000 xg for 25 minutes.

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  • A direct detection of Escherichia coli genomic DNA

    2017-1-1 · S. saprophyticus is the second most common causative agent of acute UTI in the community after Escherichia coli and is isolated mainly from urine of sexually active young women,1, 2 causing symptoms that are undistinguishable from those induced by E. coli. Cases of sepsis and pyelonephritis caused by this microorganism have also been reported.3, 4

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  • Correlation of phenotypic tests with the presence of

    2020-1-1 · Enterohemorrhagic E. coli (EHEC) and enteropathogenic E. coli (EPEC) were detected using Abs against a secreted protein (Nakasone, Toma, Lu, & Iwanaga, 2007). These cases are discussed in detail in Section 9. The advantage of LFIA is the ease of multimerization when compared to ELISA.

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  • Detection of microorganisms with lateral flow test

    Researchers at McMaster University have now developed a paper strip test that is cheap to produce, extremely portable, simple to use, and detects E. coli in water in 30 minutes. While many popular ...

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  • Separating and Detecting Escherichia Coli in a ...

    2011-8-12 · In this thesis, I present a lab-on-a-chip (LOC) that can separate and detect Escherichia Coli (E. coli) in simulated urine samples for Urinary Tract Infection (UTI) diagnosis. The LOC consists of two (concentration and sensing) chambers connected in series and an integrated impedance detector. The two-chamber approach is designed to reduce the non-

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  • The Clinical Urine Culture: Enhanced Techniques

    2016-4-25 · This percentage was only slightly better in the UTI cohort alone, where standard urine culture detected 24% (16/67) of the non-E. coli uropathogens detected by the expanded-spectrum EQUC protocol. Therefore, standard urine culture's capacity to detect E. coli dramatically skewed its overall uropathogen detection value.

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  • Bio-Sensing Dipstick Test for E. Coli in Water

    2017-9-12 · To combat such challenges in the analytical testing aspect of E. coli infections, a group of Researchers led by Dr. Naga Siva Kumar Gunda at the Waterloo Institute for Nanotechnology have constructed a new litmus paper test to detect for the presence of E. coli in water samples 2.That paper test, otherwise known as a DipTest, is composed of three different sections that include a wax ...

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  • A direct detection of Escherichia coli genomic DNA

    2012-2-6 · Preparation of spiked urine samples. The urine from normal person was spiked with uropathogenic Escherichia coli isolated from urinary tract infected patient. To prepare urine spiked clinical samples, 10 6 - 10 5 cfu/ml Escherichia coli were added to one ml of urine and centrifuged at 14000 xg for 25 minutes. The supernatant was discarded and the pellet was used for genomic DNA extraction …

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  • Correlation of phenotypic tests with the presence of

    2017-1-1 · S. saprophyticus is the second most common causative agent of acute UTI in the community after Escherichia coli and is isolated mainly from urine of sexually active young women,1, 2 causing symptoms that are undistinguishable from those induced by E. coli. Cases of sepsis and pyelonephritis caused by this microorganism have also been reported.3, 4

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  • Sensitive and rapid detection of pathogenic bacteria

    2010-1-1 · First, 30% of the participants failed to detect E. coli O157:H7, even though they were explicitly instructed to test for this organism. Second, only 49% of respondents followed a recommendation by the Association of State and Territorial Public Health Laboratory Directors to screen at least all bloody stools for E. coli O157:H7. 3

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  • Identification of Escherichia coli O157:H7 in a ...

    2010-7-27 · Most people will have already given a urine sample at some point in their lives. Urine samples are needed for urine tests, which are used for things like testing for particular diseases or monitoring their progress. For instance, urine test strips can be used to indicate whether you have a urinary tract infection.

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  • Understanding urine tests - InformedHealth.org -

    2020-1-1 · Recombinant E. coli can be used to obtain highly specific Abs. O-Ags of E. coli O157 or O145 was connected with AcrA (Castillo et al., 2017). The concatemer was used as an immunogen and highly specific Abs were produced.

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  • Detection of microorganisms with lateral flow test

    2020-6-19 · A UTI test kit may be a negative test may leave you concerned, especially if you continue to experience discomfort and other symptoms. If the test was negative, and you still have symptoms, call your doctor. You may still have an infection or there may be other medical reasons for your pain. Urinary pain can have many causes, including an STD.

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  • Disinfection of E. Coli Contaminated Urine Using

    2019-12-1 · Disinfection was then quantified using synthetic urine and E. coli as the indicator organism. The colony forming unit (CFU) count of E. Coli was reduced from >10 9 CFU/100 mL to <1000 CFU/100 mL, World Health Organization permissible limits after treatment [World Health Organization, Guidelines for the safe use of wastewater, excreta and ...

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  • COLIKAT RAPID - Enumeration of Escherichia coli and ...

    2012-2-6 · Preparation of spiked urine samples. The urine from normal person was spiked with uropathogenic Escherichia coli isolated from urinary tract infected patient. To prepare urine spiked clinical samples, 10 6 - 10 5 cfu/ml Escherichia coli were added to one ml of urine and centrifuged at 14000 xg for 25 minutes. The supernatant was discarded and the pellet was used for genomic DNA extraction …

    Get Price
  • A direct detection of Escherichia coli genomic DNA

    Researchers at McMaster University have now developed a paper strip test that is cheap to produce, extremely portable, simple to use, and detects E. coli in water in 30 minutes. While many popular ...

    Get Price
  • New paper strip test detects E. coli quickly, cheaply

    2019-5-3 · APPLICATION NOTE TaqMan Assays for urinary tract microbiota ... Real-time PCR can detect slow-growing or difficult-to-cultivate microorganisms, and can be used when ... Ba04646242_s1_E. coli Ba04932083_s1_K. pneumoniae Ba04932081_s1_. aeruginosa Ba04932076_s1_abilis

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  • Can Urine Test Detect STD | What Does a Urine Test

    2020-8-19 · Results from such a test could direct the selection of appropriate antimicrobial therapy at the point-of-care (POC). In this study, we show that a chromogenic, dual enzyme-mediated amplification system (termed DETECT [dual-enzyme trigger-enabled cascade technology]) can identify CTX-M-producing GNB from unprocessed urine samples in 30 minutes.

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  • High-throughput profiling of urinary tract microbiota ...

    2019-2-7 · To date, molecular biology techniques have been used to detect microorganisms and in epidemiological studies 30,31,32, such as multiplex PCR methods used to detect E. coli serogroups 31 .

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  • A Dual Enzyme-Based Biochemical Test Rapidly

    2020-12-22 · Nitrate-reducing bacteria, including E. coli, can switch from using oxygen to nitrate for respiration, releasing nitrite in the process. The reagent pad contains an aromatic amine compound that reacts with nitrite to produce a diazomium salt which cascades through a similar diazo coupling reaction to yield a pink dye compound.

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  • Multifunctional Paper-Based Analytical Device for In

    What hormone does a pregnancy test detect? hCG. ... When is the best time to test fertility using a urine test? 2-4 days before the estimated day of ovulation. ... Gram(-) or gram(+) E. coli bacteria. What do UTI tests detect in the urine? nitrates. What can interfere with the results of a UTI test?

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  • Rapid, Automated Detection of E.coli, coliforms &

    2021-1-5 · U-KNOW is a two-feature system, which can diagnose glucose level and auto-resetting. E. coli senses the urine for the glucose level, on the other hand, enterokinase degrades E. coli to configure the initial status for the next user. After detection, the collected data …

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  • Competition/Tracks/Diagnostics - 2020.igem.org

    2019-8-27 · of Lactobacillus, can inhibit the grown of uropathogenic bacteria, including E. coli (Aroutcheva et al., 2001; Brubaker & Wolfe, 2016). Over-prescribing antibiotics, especially in cases of asymptomatic bacteriuria, can lead to both an eradication of beneficial bacterial flora and an emergence of antibiotic-resistant bacteria.

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  • Corporate Medical Policy

    phatase, can be used to detect DNA bound to nitrocellu-lose. Biotin-labeled DNA and RNA probes have the ad-vantage of a long shelf life and are reusable in hybridization assays. We have developed a nonisotopic DNA hybridiza-tion assay capable of detecting as little as 10 pg of iso-lated CMV DNA. This report shows an application of this

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  • Detection of Cytomegalovirus in Urine by Nonisotopic

    2021-5-28 · Next, the assay was used to detect E. coli/IMP-1 and E. coli/KPC-3 (Fig. 3e and S21†). E. coli/IMP-1 was detected at 10 5 cfu mL −1 within 2 hours, and E. coli/KPC-3 could be reliably detected at around 10 5 to 10 6 cfu mL −1.

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  • A dual-caged resorufin probe for rapid screening of ...

    2020-12-22 · Nitrate-reducing bacteria, including E. coli, can switch from using oxygen to nitrate for respiration, releasing nitrite in the process. The reagent pad contains an aromatic amine compound that reacts with nitrite to produce a diazomium salt which cascades through a similar diazo coupling reaction to yield a pink dye compound.

    Get Price
  • Diagnostic hallmarks of urinary tract infection |

    2020-7-28 · Microcantilevers coated with common nutritive layers, e.g., agarose, were sensitive to cell proliferation, being able to detect active growth of E. coli cells within 1 h under temperature- and humidity-controlled conditions (Gfeller et al., 2005b). It was proposed that microbial growth promoted an increase of water absorption by the nutritive ...

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  • Antimicrobial Drug Resistance in Escherichia coli from ...

    2015-7-21 · Detect E. coli and Total Coliforms in Water with EPA Approved RAPID’E.coli™2 Agar; 5 New Features of the Whitley GMP Workstation; Latest Updates on COVID-19 Testing - Kits, Products, Services, Instrumentation - 29 June 2021; U.S. Launch of AI Based BD Kiestra™ Urine Culture Application

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  • Frontiers | Nanomechanical Sensors as a Tool for

    2020-3-4 · The produced E7 lysis protein is responsible for lysing the E. coli cells so that the generated pyocin S5 can be released into the extracellular medium to kill P. aeruginosa specifically. The results showed that the engineered E. coli can inhibit the biofilm formation by 90% and reduce the growth of planktonic P. aeruginosa by 99% [63, 64].

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  • A Novel 7-Single Nucleotide Polymorphism-Based

    2016-1-18 · The qPCR 7-SNP typing test with uidA control primers can identify E coli within 20–25 minutes and is able to clearly distinguish E coli from other members of family Enterobacteriaceae. Finally, we have demonstrated the power of the 7-SNP qPCR typing test for detection and clonotype determination of E coli directly from urine specimens.

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  • Rapid, Automated Detection of E.coli, coliforms &

    2019-2-5 · We base our trials around E coli bacteria causing urinary tract infections as these are common conditions that create high workloads for NHS laboratories (our clinical partner processes up to 1000 urine samples per day) and if improperly treated can lead to severe conditions such as sepsis.

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  • Bio-Inspired Fluorescent Carbon Dots as Probes for

    2019-8-27 · of Lactobacillus, can inhibit the grown of uropathogenic bacteria, including E. coli (Aroutcheva et al., 2001; Brubaker & Wolfe, 2016). Over-prescribing antibiotics, especially in cases of asymptomatic bacteriuria, can lead to both an eradication of beneficial bacterial flora and an emergence of antibiotic-resistant bacteria.

    Get Price
  • Corporate Medical Policy

    2015-7-21 · Detect E. coli and Total Coliforms in Water with EPA Approved RAPID’E.coli™2 Agar; 5 New Features of the Whitley GMP Workstation; Latest Updates on COVID-19 Testing - Kits, Products, Services, Instrumentation - 29 June 2021; U.S. Launch of AI Based BD Kiestra™ Urine Culture Application

    Get Price
  • Detection of pathological biomarkers in human

    Hence, 2 ml urine was fortified with 50 ng of the internal stan-dards d 3-THC, d 3-11-OH-THC, d 3-CBD, d 3-CBN, and 17α-methyltestosterone. The samples were buffered to pH 7.0 with 0.75 ml 0.8 M phosphate buffer (Na 2HPO 4:NaH 2PO 4, 1:2, w:w). Twenty-five microliters β-glucuronidase from E. coli were added. The mixture was incubated at 50 C ...

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  • Detection of Salmonella enterica in 375g Poultry in

    2015-6-22 · Luciferin can then be detected in the urine using a simple assay; with as little as 1 microliter of urine, the test was able to detect the presence of the bioluminescent luciferin (1). Using this method, the team were able to detect metastatic tumors within 24 …

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  • Preliminary data on the potential for unintentional ...

    2021-6-10 · A bilirubin in urine test measures the levels of bilirubin in your urine. Bilirubin is a yellowish substance made during the body's normal process of breaking down red blood cells. Bilirubin is found in bile, a fluid in your liver that helps you digest food. If your liver is healthy, it will remove most of the bilirubin from your body.

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  • “Friendly” Bacteria Help Diagnose Disease

    2020-3-4 · The produced E7 lysis protein is responsible for lysing the E. coli cells so that the generated pyocin S5 can be released into the extracellular medium to kill P. aeruginosa specifically. The results showed that the engineered E. coli can inhibit the biofilm formation by 90% and reduce the growth of planktonic P. aeruginosa by 99% [63, 64].

    Get Price
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