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  • Rapid and Simple Detection of Escherichia coli by Loop ...

    2020-8-9 · Urine specimens collected from patients referring to Resalat Hospital in Iran. E. coli (ATCC 25922) was used to evaluate the specificity and sensitivity of the LAMP reaction and grown in LB broth medium. DNA extraction 1.5 ml of urine was poured into a microfuge tube. The tubes were centrifuged at 14000 rpm for 10 min.

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  • Microfluidic smartphone quantitation of Escherichia

    2019-12-1 · For full response curves, a serial dilution of E. coli K12 range from 10 8 to 10 0 CFU/mL in 3% w/w BSA or synthetic urine were loaded into a Nunc maxisorp microplate 400 μL well and 3% w/w BSA used as negative control. The MCF strip was then moved to a new well loaded with 40 μg/mL biotinylated polyclonal detection antibody (detAb) and ...

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  • Detection of Escherichia coli with Fluorescent Labeled ...

    An E. coli detection system using E. coli-specific bacterio-phage T4 was constructed (11). To facilitate E. coli detection, a T4e-phage that did not produce lysozyme was constructed, and green fluorescent protein (GFP) was displayed on T4e-small outer capside (SOC) protein. This T4e-/GFP can detect E. coli K12 without cell lysis.

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  • Sample Size and Matrix Effect on the Ability of Rapid ...

    The results provide guidance on the use of modified enrichment medias, and the length of time needed to detect E. coli O157:H7 by different tests in different sizes of samples. All the detection methods accurately detected E. coli O157:H7 in all the different types of samples except for offal samples that contained liver.

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  • Development of a fluorescent distance-based paper

    The highly sensitive and selective determination of Escherichia coli (E. coli) in urine was achieved using a SYBR™ safe loop-mediated isothermal amplification (LAMP) method with a distance-based paper device. New primers set specific to multi-copy the 16s rRNA gene of E. coli were designed and used in this s

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  • Multiplex PCR Based Urinary Tract Infection (UTI)

    2020-2-1 · E. coli was also the most common bacterium detected by culture (34% of culture positives, 74/217) and Enterococcus faecalis was the second most common (21% of culture positives, 46/217). Download : Download high-res image (452KB) Download : Download full-size image; Figure 1.

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  • Biosensors for detection of pathogenic bacteria ...

    1999-10-1 · E. coli O157:H7 is a rare strain of E. coli that is considered to be one of the most dangerous foodborne pathogens (Griffin and Tauxe, 1991, Buchanan and Doly, 1997). This O157:H7 strain produces large quantities of a potent toxin, in the lining of the intestine, and causes severe damage resulting in hemorrhagic colitis or hemolytic uremic ...

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  • Causes and treatment of E. coli in the urine of a child ...

    2021-6-24 · Specificity of the e-RCD assay. After confirming that the e-RCD assay could sensitively detect E. coli in both buffer and urine, we assessed the specificity of this assay against a panel of Gram ...

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  • Integrating programmable DNAzymes with electrical

    2020-1-1 · Enterohemorrhagic E. coli (EHEC) and enteropathogenic E. coli (EPEC) were detected using Abs against a secreted protein (Nakasone, Toma, Lu, & Iwanaga, 2007). These cases are discussed in detail in Section 9. The advantage of LFIA is the ease of multimerization when compared to ELISA.

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  • Urinary tract infection: detection of Escherichia coli ...

    The hyperimmune serum reacted with all urine specimens studied. However, bands of 77, 66, 51, 43, and 21 kDa were detected in urine specimens positive for E. coli. The urine specimens that were positive for heterologous bacteria showed one to four bands, which were combination of …

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  • Quantitative detection of Escherichia coli from urine

    2012-12-1 · Quantification of E. Coli in Urine Specimens and Comparison of Results. We quantified the copy numbers of E. coli from urine specimens using the standard curve described above. In all the urine samples in which E. coli was detected, we detected 5.9 × 10 7 to 1.2 × 10 2 copies/mL of E. coli.

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  • A direct detection of Escherichia coli genomic DNA

    2012-2-6 · Preparation of spiked urine samples. The urine from normal person was spiked with uropathogenic Escherichia coli isolated from urinary tract infected patient. To prepare urine spiked clinical samples, 10 6 - 10 5 cfu/ml Escherichia coli were added to one ml of urine and centrifuged at 14000 xg for 25 minutes. The supernatant was discarded and the pellet was used for genomic DNA extraction as ...

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  • Laboratory testing for biomarkers of alcohol exposure

    2021-1-6 · Non-volatile, water-soluble, direct minor metabolite of ethanol Excreted in the urine Appears 1 h after ethanol ingestion ... Hydrolysis of EtG by E. coli in urine specimens Helander and Dahl, 2005. Phosphatidylethanol ... urine •Not able to detect methanol and isopropanol overdoses CH …

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  • Shiga toxin-producing Escherichia coli (STEC)

    2018-6-27 · 1 Shiga toxin-producing Escherichia coli (STEC) Escherichia coli are bacteria that form part of the normal gut flora of humans and other warm-blooded animals. Although most E. coli are considered harmless, certain strains can cause severe illness in humans, particularly Shiga toxin-producing E. coli (STEC). Infection

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  • Rapid detection of respiring Escherichia coli O157:H7

    Respiring E. coli O157:H7 and E. coli K-12 were inoculated in food samples as 10 6 cells/ml (apple juice and milk) or 10 6 cells/g (ground beef). E. coli cells in apple juice (A), apple juice after centrifugation (B), milk with fat removal and proteinase treatment (C), and ground beef after clarifying (D) were detected after FA-CTC double staining.

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  • Urine-based antigen detection assay for diagnosis of ...

    2020-4-23 · Author summary Visceral leishmaniasis (VL) or kala-azar is a systemic parasitic disease that is endemic in 75 countries with more than 500 million people at risk of infection. VL is caused primarily by the parasites known as Leishmania infantum and Leishmania donovani. It is estimated that VL affects 50,000–90,000 people each year, with 90% of the cases occurring in India, Kenya, Somalia ...

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  • PREVENTING E.coli in FOOD Management Framework

    2011-6-23 · Escherichia coli (E. coli) is a bacterium found in the digestive tract of animals and humans. Generally. harmless, some E. coli are pathogenic and can contaminate food, water and the environment. Hundreds of thousands of people. are made ill by E. coli each year, with hundreds of them dying. In recent years, there has been an

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  • Characterization of Ampicillin Resistant Gene (blaTEM-1 ...

    2020-7-3 · Characterization of Ampicillin Resistant Gene (blaTEM-1) Isolated from E. coli in Northern Jordan Fatima-Azzahra Delmani*1, Adnan S. Jaran 2, Yaser Al Tarazi3, Hani Masaadeh4 and Omar Zaki2 1Department of Biology, Faculty of Science, Jerash University, Jordan 2Department of Biological Science, Al al-Bayt University, Mafraq, Jordan 3Basic Department of Veterinary Medical Sciences …

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  • Identification of Pathogens by Mass Spectrometry ...

    2010-4-1 · Fig. 3 presents MALDI mass spectra obtained from the direct analyses of intact bacterial cells of 4 pure cultures including S. aureus, Enterococcus faecalis, E. coli, and Vibrio parahaemolyticus. Because the protein profiles clearly differ, they can be used to differentiate these 4 bacteria.

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  • A direct detection of Escherichia coli genomic DNA

    2012-2-6 · Preparation of spiked urine samples. The urine from normal person was spiked with uropathogenic Escherichia coli isolated from urinary tract infected patient. To prepare urine spiked clinical samples, 10 6 - 10 5 cfu/ml Escherichia coli were added to one ml of urine and centrifuged at 14000 xg for 25 minutes. The supernatant was discarded and the pellet was used for genomic DNA extraction as ...

    Get Price
  • Researchers Develop Sensor to Test for E coli in 10 ...

    2019-4-10 · Applicability of routine analytical procedures to detect andarine and ostarine. A comparative study Antidoping Centre, Moscow, Moscow, Russia ... 3 mL of urine were deconjugated with a solution of β-glucuronidase from E.coli in phosphate buffer containing ... direct urine analysis, while giving a much deeper insight into the metabolism as it ...

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  • Applicability of routine analytical procedures to detect ...

    munity origin of the UTI. In our setting, Escherichia coli is the most frequent pathogen detected in UTIs in both hospital and community samples (Sorlozano et al. 2014). The gold standard for UTI diagnosis is urine culture to identify and quantify uropathogens followed by evalua-tion of their antibiotic susceptibility.

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  • Comparison between urine culture profile and

    Subsequently, human urine samples spiked with E. coli were incubated with Man-CQDs. The photostability of the fabricated formulation and the unique fluorescent properties of QDs enabled the successful labeling and detection of E. coli in all samples with a minimum detectable concentration of 10 3 CFU mL −1. Even though, the proposed method ...

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  • Interpretation of Urine Culture Colony Counts ...

    2010-4-1 · Fig. 3 presents MALDI mass spectra obtained from the direct analyses of intact bacterial cells of 4 pure cultures including S. aureus, Enterococcus faecalis, E. coli, and Vibrio parahaemolyticus. Because the protein profiles clearly differ, they can be used to differentiate these 4 bacteria.

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  • Nanotools for Sepsis Diagnosis and Treatment ...

    2021-3-1 · Enrichment protocols also may fail to detect strains of bacteria present in the food if they are at low levels. A closer observation at methods available for the detection of organisms like total coliform and E. coli reveal that, majority of these methods have incubation at 37 °C for 24 h to obtain the results. Hence, researchers are exploring ...

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  • Applicability of routine analytical procedures to detect ...

    2019-4-10 · Applicability of routine analytical procedures to detect andarine and ostarine. A comparative study Antidoping Centre, Moscow, Moscow, Russia ... 3 mL of urine were deconjugated with a solution of β-glucuronidase from E.coli in phosphate buffer containing ... direct urine analysis, while giving a much deeper insight into the metabolism as it ...

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  • Researchers Develop Sensor to Test for E coli in 10 ...

    tomatic UTI enrolled from research hospitals, E coli was the most frequent pathogen identified in men and women, accounting for 49.7% of the total isolates. Other pathogens identified were S epidermidis (9.1%), E faecalis (8.6%), K pneumoniae (5.1%) and P mirabilis (3.4%). The results obtained in this study are similar to those of other studies

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  • Open Access Research Characteristics of urinary tract ...

    2018-10-26 · Prior to lipid A extraction, these 5 strains pellets were resuspended in 1 mL sterile urine individually. E. coli was mixed with antibiotic-susceptible or -resistant A. baumannii or K. pneumoniae ...

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  • Pathogen Identification Direct From Polymicrobial ...

    2021-3-1 · Enrichment protocols also may fail to detect strains of bacteria present in the food if they are at low levels. A closer observation at methods available for the detection of organisms like total coliform and E. coli reveal that, majority of these methods have incubation at 37 °C for 24 h to obtain the results. Hence, researchers are exploring ...

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  • Laboratory Diagnosis of Urinary Tract Infections in

    2020-11-2 · The application of next-generation molecular, biochemical and immunological methods for developing new vaccines, antimicrobial compounds, probiotics and prebiotics for zoonotic infection control has been fundamental to the understanding and preservation of the symbiotic relationship between animals and humans. With increasing rates of antibiotic use, resistant bacterial infections have …

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  • Rapid diagnostic assay for detection of cellulose in

    2007-7-30 · Kilian and Bülow (1979) described a chromogenic medium that employed a substrate for β-glucuronidase, thus allowing the direct detection of the commonest pathogen of the urinary tract, Escherichia coli. In a large study using 9247 urine samples, the authors reported that 94% of E. coli strains could be identified by their coloured appearance ...

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  • The application of chromogenic media in clinical ...

    Overall, 21 urine samples were included in this study. All of them were culture positive on MacConkey agar plates, with more than 10 6 E. coli CFU/ml urine in pure cultures, and microscopic inspection of urine sediments revealed the presence of massive numbers of neutrophils (>100/µl). The 21 patients were mainly elderly (mean age above 60 ...

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  • In Vivo mRNA Profiling of Uropathogenic Escherichia

    2021-7-9 · For example, Pyle et al. (1995) combined incubation using cyanoditolyl tetrazolium chloride (CTC) to detect respiratory activity with a modified FA technique, for the enumeration of viable E. coli O157:H7 in water in three to four hours. Bacteria were captured by filtration on nonfluorescent polycarbonate membranes, incubated on absorbent pads ...

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  • E. Coli under the Microscope -Types, Techniques,

    2000-8-1 · These laboratories cover approximately 14% of the Dutch population (15.4 million inhabitants in 1994). They tested >75% of the E. coli isolates for resistance to amoxycillin, trimethoprim, nitrofurantoin and norfloxacin. Only E. coli isolates from urine were included. Repeat isolates of E. coli from the same patient taken within 2 months were ...

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  • 9 Methods to Identify and Detect Microbial

    Adaptable microfluidic system for single-cell pathogen classification and antimicrobial susceptibility testing Hui Lia, Peter Torabb, Kathleen E. Machc, Christine Surretted, Matthew R. Englande, David W. Crafte, Neal J. Thomasf,g, Joseph C. Liaoc, Chris Puleod, and Pak Kin Wonga,b,h,1 aDepartment of Biomedical Engineering, The Pennsylva nia State University, University Park,PA 16802 ...

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  • New solutions to capture and enrich bacteria from

    2021-7-9 · Urine test strips are packaged along with a drying agent in a plastic bottle with a twist-off cap. Each strip is stable and ready to use upon removal from the bottle. The entire test strip is disposable. Results are obtained by direct comparison of the test strip with the color blocks printed on the bottle label; or by our urine analyzer.

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  • Increasing resistance to fluoroquinolones in

    2019-5-8 · The prevalence of ESBL-producing E. coli based on MIC in human, animal, and environmental/food isolates was 42.4%, 63.2%, and 28.6%, respectively. The prevalence of ESBL-producing E. coli based on the DDM in human, animal, and environmental/food isolates was 13%, 26.3%, and 25%, respectively. The prevalence of ESBL antibiotic resistance in ...

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